The image analyses including 3D rendering, colocalization, and FRAP can be performed qualitatively and quantitatively with Slidebook 5.0 software. The microscope is also equipped with a live cell environmental chamber that maintains the temperature and CO2 level constant. This system has a precise high-speed laser scan head for FRAP, FLIP, photoconversion, and photoactivation analyses. With 4 lasers (405, 488, 561, and 640 nm), users can acquire high resolution multicolor images in 4D. This system is equipped with motorized xy stage movement and fast piezo z focusing module that allow users to monitor multiple regions of interest automatically during live imaging. Fast scanning and highly sensitive EMCCD camera on this system minimizes phototoxicity to living cells and bleaching of samples without sacrificing fluorescence detection, which is critical for long term live cell imaging. This system consists of an Olympus IX81 inverted microscope equipped with the 3i Yokogawa spinning disk scanner and CCD cameras that allow fast high-resolution image acquisition with revolving laser arrays.
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